Synapt g2s high definition mass spectrometer from waters. Waters synapt hdms system enables the analysis of samples differentiated by size and shape, as well as mass, to deliver increased specificity and sample definition beyond that achievable by conventional mass spectrometers. No communication with the epc in zq, quattro micro, zmd, or emd wkb2825. Comprehensive and reproducible identification and labelfree quantification of proteins. Synapt g2 hdms is a high resolution exact mass msms platform designed to get you to the right result, faster no matter how challenging your application is. Synapt g2s hdms is a high resolution exact mass msms platform designed to get you to. Synapt g2si mass spectrometry from waters corporation. The waters synapt g2s high definition mass spectrometer hdms is used for discovery and targeted omicbased experiments. I am looking for other users of a waters nacquity uplc coupled to a synapt g2si running hdmrm and hdmsms measurements of peptides and. Protein summary display accenting the protein and peptides of interest, peptide. Synapt g2 high definition mass spectrometer duke gcb. Transforming your capacity for discovery, understanding, and success. Waters introduces synapt g2s mass spectrometer, combining revolutionary stepwave ion optics with ion mobility technology taking performance to new level. Visualization of the raw data in 3dmontage view is shown in.
Visualization and comparison of synapt g2s lcms data. Ms detector is not present in console, and the control panel is unavailable in empower wkb2858. The waters guys have provided me with mascot mgf peak list files, but what i would really like. Waters synapt g2 hdms 4kda or 8kda myco analytical.
Synapt g2s hdms waters the mclean group laboratory for. Waters synapt g2si tof department of chemistry and. Synapt g2s combines revolutionary high performance stepwave ion. Considerations specific to the maldi synapt g2si system laser radiation hazard the maldi synapt g2si system uses a solidstate laser to produce a concentrated beam of invisible uv radiation. Designed for leading researchers working at the limits of conventional mass spectrometery capabilities who need to further characterize and define their samples, waters synapt tm hdms tm system offers unique, enabling functionality waters synapt hdms tm system enables analysis of samples differentiated by size and shape. If the signal is too low increase the detector voltage to reach signal in mid e 6. There is no need to op en the inlet configuration wizard. You should be able to by a synapt g2 without the ion mobility turned on for a bit of a discount and then decide later if it is something you need. Two inlet options are currently available for the university of windsor synapt, nanoelectrospray ionization nesi and matrix assisted laser desorption ionization maldi. In addition, g2 could go up to higher pressure than g1. Available from krss a used fully refurbished and service waters synapt g2 system with control pc and optional nanoacquity. Waters synapt g2si hdms the synapt g2si high definition mass spectrometer from waters is a hybrid quadrupole, ion mobility, orthogonal acceleration timeofflight oatof mass spectrometer that combines high resolution mass spectrometry with high efficiency ion mobilitybased measurements and separation ims.
Consequently, there is also no difference between the chromatogram for the precursor and for the product. Refurbished waters synapt g2 complete with esi source, pumps and masslynx 4. With highefficiency twave ion mobility, you can use an additional dimension of separation, based on molecular size and shape. The maldi synapt g2si is a class 1 laser product, as classified to the requirements of eniec 608251. If the signal is too high and shows artefact peaks after the real ones decrease the detector voltage to remove artefacts. The bsa oncolumn amounts ranged from one attomole to one femtomole oncolumn. The waters synapt g2si system is utilized for protein applications such as proteinprotein interaction experiments. Synapt g2si mass spectrometer, with a mass range up to 100,000 on mz scale in resolution mode, provides unique separation capability of highly complex samples in addition to hplc by its advanced twave ionmobility technology. By combining stepwave plus quantof and triwave technologies, it provides a step change in ms performance. Synapt g2s combines revolutionary stepwave ion optics with proven quantitative tof.
Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% lc eluate. It has an electrospray ionization esi interface, meaning that analytes need to be dissolved in mixture of aqueousorganic solution e. The nanoacquity uplc system includes a binary solvent manager. Is it possible to convert waters synapt g2si raw data to the mzml. I will say for the g2s, that it is significantly more sensitive than the g2 when we looked at that one. Synapt g2 ms is a high resolution exact mass msms platform designed to get you to the right result, faster no matter how challenging your application is whether you specialize in metabolite profiling, proteomics, biomarker discovery. Synapt g2si high definition ms system specifications. The waters synapt g2 hdms is a refurbished 2010 high resolution exact mass msms platform that provides a new dimension of performance to drive scientific discovery. Synapt delivers a third dimension to your analysis, proven to transform your analytical perspective whatever the application. Through highefficiency twave ion mobility you can get superior separation, enhance the peak capacity, specificity and sensitivity of your. Synapt g2s enables a mixture of two reverse sequence peptides grgds and sdgrg differing in ccs. Synapt g2 s i ms and hdms epc reset occasionally after a reboot of the synapt g2 s i. Waters synapt g2si high definition mass spectrometry center.
Weve combined quantof tm breakthrough quantitative tof technology and enhanced high definition ms tm techn ologies. Experiment principle high definition multi reaction monitoring acquisition. Sometimes chromatography and mass resolution are not enough. Synapt g2s hdms is a mass spectrometer that uses the extra dimension of ion mobility separations to maximize selectivity and confidence in results.
Rapid high mass resolution mass spectrometry using matrix. The instrument is a class 1 laser product, as classified by en 608251. Considerations specific to the synapt g2 ms system solvent leakage hazard the source exhaust system is designed to be robust and leaktight. Synapt g2 is a qtof hybrid instrument, mainly used for small molecule mass analysis and hydrogendeuterium exchangemass spectrometry hdxms. Synapt g2 laboratory equipment pdf manual download. Features quantitative quadrupole timeofflight mass spectrometer quantoftm high resolution exact msms travelling wave ion. Learn about the benefits of ion mobility separations ims available with the synapt. After separation of the ions based on their ionmobilities in the imscell utilizing a traveling. The solution a 100ng escherichia coli sample was injected oncolumn. Synapt g2, our secondgeneration platform, is the product of our very latest innovations.
Synapt g2s hdms waters the mclean group laboratory. Waters introduces synapt g2s mass spectrometer, combining. Waters synapt g2 for sale used, price buy from cae. Lastly, g2 could operate in higher traveling wave voltage and has different repeat pattern when. Features quantitative quadrupole timeofflight mass spectrometer quantoftm high resolution exact msms travelling wave ion mobility separation triwavetm cell, incl. The second generation synapt tm g2 platform from waters corporation provides a new dimension of performance to drive your scientific discovery like never before. The waters synapt g2si timeofflight mass spectrometer tof ms is a sophisticated high resolution mass spectrometer capable of a wide range of measurements. Ryan oftebro is a pharmacist and principalceo of kelleyross pharmacy group, which consists of five divisions located in seattle, wa, including compounding pharmacy, community pharmacy, long term care, specialty pharmacy, and a clinical division dedicated to developing and implementing innovative pharmacy services.
Schematic view of the waters synapt g2s hdms instrument platform equipped with a travelingwave ionmobility cell. You may be prompted for some additional configuration information when first opening the console. The synapt g2s system provides a unique platform to further your discovery efforts by expanding on the capabilities of conventional ms instrumentation. Analyses were performed on waters synapt g2 manchester, uk and.
The masslynx calibration is imported into unifi as a unifi report file. Synapt g2 and g1 both have similar instrument schematic except few differences in the ims cell. Waters synapt g2 hdms shared instrumentation network. Make sure, that leuenk is still giving strong signal. Synapt high definition mass spectrometry is the combination of highefficiency ion mobility measurements and separations with highperformance tandem ms, enabling the differentiation. See unifi help files for details on how to do this in masslynx, print the masslynx intellistart calibration report, selecting the unifi printer method as the printer.
Considerations specific to the maldi synapt g2 hdms system laser radiation hazard the maldi synapt g2 hdms system uses a solidstate laser to produce a concentrated beam of invisible uv radiation. The peptides were separated and analyzed using a nanoacquity uplc system coupled with a synapt g2 s operating at a precursor and product ion mass resolution of 20,000 fwhm, and data acquired in lchdms e scanning mode. The unique ability to use the collision cross section. Synapt g2si high definition ms system uses the extra dimension of ion mobility separations to maximize selectivity and confidence in results. Trap twave, ims twave, transfer twave stepwavetm ion optics video overview of the stepwavetm technology. Find the best deals on used waters synapt g2, or send us a request for an item and we will contact you with matches available for sale. Catalog number maldi synapt g2si this product is no longer available on biocompare.
Synapt g2si high definition ms system uses the extra dimension of ion mobility separations to maximize selectivity and confidence in. The synapt g2 system features quantof technology that combines an innovative high field pusher and dual stage reflectron with a novel ion detection system in an optimised, folded, tof geometry. This document includes system hardware and performance specifications. The instrument incorporates the newest technologies in the area of ion optics and ion mobility separation, with interchangeable universal ion source architecture, allowing. Uplc synapt g2 ms nanouplcmsms system the nanoacquity uplc synapt g2 ms nanouplcmsms system includes a nanoacquity uplc system and the waters synapt g2 ms fitted with the nanolockspray source. Detector setup fails with beam error wkb12269 waters. Waters introduces the synapt g2si to its mass spectrometer product line instrument is the most advanced ion mobilityenhanced research mass spectrometer. This hdms platform provides accurate mass measurements, multiple tandem mass spectrometry workflows, and ion mobility separation of complex mixtures. Synapt g2si hdms redefining resolution resolution in 3 dimensions. Skyline seems to analyze all three datas at the same time, and show one combined chromatogram.
The water synapt g2 raw data contains three data files function1 for ms1, function2 for msms, and function3 for lock mass. Waters synapt g2si quadrupoleion mobilitytof hybrid instrument serves a broad range of applications by providing the most extensive range of targeted data acquisition. The waters synapt g2 is ideal for qualitative and more recently combined qualitative and quantitative workflows. Synapt g2si uses high efficiency twave ion mobility to significantly enhance the peak capacity, specificity and sensitivity of your analysis. High definition hybrid quadrupoletimeofflight mass spectrometer. When you want to reach beyond the boundaries of conventional mass spectrometry, synapt g2 high definition mass spectrometry hdms provides a new dimension to your discovery. Its synapt g2s instrument and it has ion mobility datadrift time. Mass spectrometers and other used analytical instruments. If you are familiar with them and able to visit our facility you welcome to test it on your own.
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